Coding

Part:BBa_K1696008

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-11)

RcfB expression cassette

RcfB plays a important role in acid adaptation by binding the DNA target site, ACiD-box, in P170 which then will be activated with the participation of acid. As far as we know, P170 has a pretty limited sensitivity that is around pH 6.0~6.5. To increase the sensing range of P170, it is practical to think about boosting rcfB generation. Accordingly, we intend to construct a strong promoter and a strong RBS into the system for a higher rcfB protein expression level.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36
    Illegal BsaI.rc site found at 764


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Parameters
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